The axenic culture of L. rhinocerotis (KUM61075) was obtained from the Mushroom Research Centre, University of Malaya.The sclerotium of L. rhinocerotis was produced by solid-substrate fermentation of mycelium on agroresidues according to the method of Abdullah et al. Harvested sclerotium was washed with distilled water and dried in the oven at 40ํ ํC for 3-5 days. The glucose-yeast extract-malt extract-peptone (GYMP, Oxoid, Hampshire, UK) medium was used for liquid fermentation. Flasks were inoculated with mycelial plugs and incubated at 25 ํC under static conditions or placed on a reciprocal shaker at 150 rpm. After 15 days, the cultures were harvested; mycelium was filtered off from the culture broth and repeatedly washed with distilled water. Mycelium and culture broth were freeze-dried and kept in air-tight containers at -20 ํC.
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